Basic methods in diagnostic molecular pathology

Basic methods in diagnostic molecular pathology

In situ hybridisation H. In situ hybridisation (ISH) uses a labelled oligonucleotide probe - that targets a specific sequence of RNA or DNA. It allows visu - alisation of the presence or absence and location of a particular RNA or DNA sequence in situ in tissue sections. Visualisation may depend on autoradiography , fluorescence microscopy - or bright-field microscopy . Chromogenic in situ hybridisation (CISH) combines ISH and immunohistochemistry for the detection of specific nucleic acid sequences and is a common alternative to fluorescence in situ hybridisation (FISH) for the detection of HER2 amplification. Viral genomes, e.g. EBV - ( Figure 11.28 ), CMV and high-risk HPV types are detectable using this approach. ISH plays an important role in tissue diagnostics and the management of tumours.

Figure 11.28 In situ hybridisation for Epstein–Barr virus (EBV) showing extensive nuclear positivity (black nuclei) in an EBV-positive gastric adenocarcinoma.

Basic methods in diagnostic molecular pathology

In situ hybridisation H. In situ hybridisation (ISH) uses a labelled oligonucleotide probe - that targets a specific sequence of RNA or DNA. It allows visu - alisation of the presence or absence and location of a particular RNA or DNA sequence in situ in tissue sections. Visualisation may depend on autoradiography , fluorescence microscopy - or bright-field microscopy . Chromogenic in situ hybridisation (CISH) combines ISH and immunohistochemistry for the detection of specific nucleic acid sequences and is a common alternative to fluorescence in situ hybridisation (FISH) for the detection of HER2 amplification. Viral genomes, e.g. EBV - ( Figure 11.28 ), CMV and high-risk HPV types are detectable using this approach. ISH plays an important role in tissue diagnostics and the management of tumours.

Figure 11.28 In situ hybridisation for Epstein–Barr virus (EBV) showing extensive nuclear positivity (black nuclei) in an EBV-positive gastric adenocarcinoma.

Basic methods in diagnostic molecular pathology

In situ hybridisation H. In situ hybridisation (ISH) uses a labelled oligonucleotide probe - that targets a specific sequence of RNA or DNA. It allows visu - alisation of the presence or absence and location of a particular RNA or DNA sequence in situ in tissue sections. Visualisation may depend on autoradiography , fluorescence microscopy - or bright-field microscopy . Chromogenic in situ hybridisation (CISH) combines ISH and immunohistochemistry for the detection of specific nucleic acid sequences and is a common alternative to fluorescence in situ hybridisation (FISH) for the detection of HER2 amplification. Viral genomes, e.g. EBV - ( Figure 11.28 ), CMV and high-risk HPV types are detectable using this approach. ISH plays an important role in tissue diagnostics and the management of tumours.

Figure 11.28 In situ hybridisation for Epstein–Barr virus (EBV) showing extensive nuclear positivity (black nuclei) in an EBV-positive gastric adenocarcinoma.