Cytogenetics and fluorescence in situ hybridisation

Cytogenetics and fluorescence in situ hybridisation

Conventional cytogenetics is the microscopic study of chromo somal changes in individual cells. Newer techniques, including FISH, array comparative genomic hybridisation, RT-PCR and next-generation sequencing (NGS) are increasingly replacing conv entional cytogenetics. Cytogenetic tests seek alterations such as gene amplification, loss of segments of chromosomal material, loss of whole chromosomes (e.g. in renal cell carci noma) and translocations with associated fusion genes (e.g. EWSR1-FLI1 in Ewing’s sarcoma). Cytogenetics and fluorescence in situ hybridisation

Conventional cytogenetics is the microscopic study of chromo somal changes in individual cells. Newer techniques, including FISH, array comparative genomic hybridisation, RT-PCR and next-generation sequencing (NGS) are increasingly replacing conv entional cytogenetics. Cytogenetic tests seek alterations such as gene amplification, loss of segments of chromosomal material, loss of whole chromosomes (e.g. in renal cell carci noma) and translocations with associated fusion genes (e.g. EWSR1-FLI1 in Ewing’s sarcoma). Cytogenetics and fluorescence in situ hybridisation

Conventional cytogenetics is the microscopic study of chromo somal changes in individual cells. Newer techniques, including FISH, array comparative genomic hybridisation, RT-PCR and next-generation sequencing (NGS) are increasingly replacing conv entional cytogenetics. Cytogenetic tests seek alterations such as gene amplification, loss of segments of chromosomal material, loss of whole chromosomes (e.g. in renal cell carci noma) and translocations with associated fusion genes (e.g. EWSR1-FLI1 in Ewing’s sarcoma).